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American Society of Microbiology ABMM Sample Questions:
1. A clinical microbiology laboratory is validating a new automated blood culture system. To assess the system's ability to detect true positive results, they should:
A) Evaluate the system's performance with samples from patients with known bloodstream infections.
B) Process a large number of sterile blood samples.
C) Spike known concentrations of various microorganisms into blood culture bottles.
D) Compare the time to detection with the current manual blood culture method.
2. A patient who received a solid organ transplant develops a pneumonia. Bronchoalveolar lavage fluid is positive for a single-stranded RNA virus that can cause giant cell formation with intranuclear and intracytoplasmic inclusions. The MOST likely causative agent is:
A) Adenovirus
B) Respiratory syncytial virus (RSV)
C) Parainfluenza virus
D) Human metapneumovirus
3. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:
A) A positive control containing Neisseria gonorrhoeae DNA.
B) A high concentration of the Chlamydia trachomatis target sequence.
C) An internal amplification control to detect PCR inhibition.
D) A melting curve analysis to confirm the specificity of the amplicon.
4. A patient with a history of neutropenia develops a severe sinus infection. Tissue biopsy reveals broad, ribbon- like, non-septate hyphae with right-angle branching. The MOST likely causative organism belongs to the order:
A) Onygenales
B) Aspergillales
C) Mucorales
D) Eurotiales
5. A clinical microbiology laboratory is using Sanger sequencing to identify mutations associated with antibiotic resistance in Mycobacterium tuberculosis. This method directly determines the:
A) Nucleotide sequence of the target gene.
B) Minimum inhibitory concentration (MIC) of the antibiotic.
C) Relative abundance of different M. tuberculosis strains in a mixed infection.
D) Presence or absence of specific resistance genes.
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: C | Question # 3 Answer: C | Question # 4 Answer: C | Question # 5 Answer: A |



